بررسی برون تنی عصاره‌ی هیدروالکلی سیر وحشی (Allium ampeloprasum) بر فعالیت حرکتی ایلئوم موش صحرایی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 کارشناس ارشد، مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

2 دانشجوی پزشکی، مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

3 استاد، گروه نفرولوژی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

4 کارشناس ارشد، گروه آمار، دانشکده‌ی بهداشت، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

5 استاد، مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران

چکیده

مقدمه: در این تحقیق، اثر ضد انقباضی عصاره‌ی هیدروالکلی برگ گیاه تره‌ی کوهی بر انقباضات ایلئوم موش‌های صحرایی نژاد ویستار و مکانیسم محتمل بر آن بررسی شد.روش‌ها: در این تحقیق مداخله‌ای تعداد 48 سر موش صحرایی، از نژاد ویستار به طور تصادفی به شش گروه هشت‌تایی به شرح زیر تقسیم شدند: شاهد، دریافت کننده‌ی غلظت‌های تجمعی عصاره‌ی تره‌ی کوهی، دریافت کننده‌ی L-NAME (L-NG-nitroarginine methyl ester) و عصاره، نالوکسان و عصاره، پروپرانولول و عصاره و گروه دریافت کننده‌ی غلظت‌های تجمعی کلرور کلسیم. در روز آزمایش، ایلئوم موش‌ها جدا و انقباضات آن در حمام بافت تحت یک گرم کشش با اضافه کردن کلرور پتاسیم (mM 60) به روش ایزوتونیک ثبت شد. جهت بررسی مکانیسم اثر عصاره، بافت با پروپرانول، نالوکسان و یا L-NAME انکوبه شد و درصد تغییرات نیروی انقباضی بر روی کاغذ ثبت و محاسبه گردید. همچنین جهت نقش کانال‌های کلسیم در فعالیت حرکتی بافت، ایلئوم تحت تأثیر غلظت‌های تجمعی کلرور کلسیم قرار داده شد.یافته‌ها: عصاره‌ی تجمعی تره‌ی کوهی (100، 200 و 400 میلی‌گرم بر کیلوگرم) به صورت وابسته به غلظت انقباضات ایلئوم به وسیله‌ی کلرور پتاسیم را کاهش داد (0001/0 > P). پروپرانولول اثر مهاری عصاره بر انقباضات ناشی از کلرور پتاسیم را به صورت معنی‌داری کاهش داد (0010/0 > P). اما، L-NAME و نالوکسان سبب کاهش اثر مهاری عصاره بر ایلئوم نشدند. همچنین کلسیم سبب انقباض بافت دپولاریزه شده توسط کلرور پتاسیم شد و این اثر انقباضی توسط غلظت‌های تجمعی عصاره به طور معنی‌داری کاهش یافت (0010/0 > P).نتیجه‌گیری: عصاره‌ی هیدروالکلی برگ تره‌ی کوهی توانست با اثر بر گیرنده‌های بتاآدرنرژیک و کانال‌های کلسیم وابسته به ولتاژ، بر فعالیت حرکتی ایلئوم موش صحرایی اثر بگذارد و با توجه به نتیجه‌ی اثر، ممکن است بتوان از آن در درمان ناراحتی‌های گوارش استفاده کرد.

کلیدواژه‌ها

عنوان مقاله [English]

In-Vitro Evaluation of Hydroalcoholic Extract of Allium Ampeloprasum on Rat Ileum Contractions

نویسندگان [English]

  • Masoumeh Sedighi-Hafshejani 1
  • Mosayyeb Noori-Ahmadabadi 2
  • Hamid Nasri 3
  • Molok Hadi 4
  • Mahmoud Rafieian-Kopaei 5
1 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Student of Medicine, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Professor, Department of Nephrology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Department of Statistics, School of Health, Shahrekord University of Medical Sciences, Shahrekord, Iran
5 Professor, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
چکیده [English]

Background: Ampeloprasum is traditionally used for gastrointestinal disorders. In this experimental study, the effect of ethanolic extract of Allium ampeloprasum was investigated on the rat ileum contractions. The mechanisms were also evaluated.Methods: In this experimental study, 48 male Wistar rats were randomly designated into six equal groups, including control and cumulative concentrations of Allium ampeloprasum, propranolol, naloxone, L-name, and CaCl2. In the day of the experiment, the ileum was dissected and its contractions were recorded in Tyrode solution in an organ bath. To evaluate the mechanisms involved, propranolol, naloxone, or L-name was incubated and the percent of changes in contractions were recorded.Findings: The extract of Allium ampeloprasum dose-dependently reduced the contractions induced by KCl (60 mM) (P < 0.0001); propranolol reversed this effect (P < 0.0010), but L-name and naloxone were not effective. CaCl2 increased the contractions (P < 0.0010) and the extract reversed this effect.Conclusion: Allium ampeloprasum leaves can affect the ileum contractions through beta adrenoreceptors and voltage-dependent calcium channels and might be used in gastrointestinal disorders.

کلیدواژه‌ها [English]

  • Allium ampeloprasum
  • Ileum
  • Beta-blockers

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مجله دانشکده پزشکی اصفهان
دوره 31، شماره 268 - شماره پیاپی 268
بهمن و اسفند 1392
صفحه 2238-2249

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  • تاریخ دریافت: 20 تیر 1392
  • تاریخ بازنگری: 06 اردیبهشت 1403
  • تاریخ پذیرش: 17 فروردین 1401

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