Journal of Isfahan Medical School

Journal of Isfahan Medical School

A Study of the Prevalence and Antibacterial Resistance of Coagulase Negative Staphylococci in Keratitis Infections following the use of Soft Contact Lenses

Document Type : Original Article (s)

Authors
Golfam Oryan 1
Jamshid Faghri 2
Hossein Fazeli 2
Alireza Zandi 3
Nafiseh-Sadat Hosseini 4
Mansour Sedighi 1
Safora Heidari 5
1 MSc Student, Department of Microbiology and Virology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
2 Associate Professor, Department of Microbiology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Department Optalmology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 PhD Student, Department of Microbiology and Virology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
5 Department of Management of Health Care Services, Shahid Motahari Hospital, Isfahan, Iran
Abstract
Background: This study aimed to determine the prevalence and antibacterial resistance of coagulase negative staphylococci in keratitis infections after using soft contact lenses in patients admitted at Feiz hospital, Isfahan Iran, in 2013.Methods: In this study, 77 patients with keratitis were examined. The samples were cultured and isolation of coagulase negative staphylococcus was done using phenotypic tests and in-vitro sensitivity testing was done using Kirby-Bauer disc diffusion susceptibility method.Findings: 38 isolates were conveniently identified as coagulase negative staphylococci. 27 samples (71.1%) were resistant to penicillin, 21 (55.3%) to erythromycin, and 16 (42.1%), to tetracycline. All the isolates (100%) were sensitive to gentamicin, 36 isolates (94.7%) were sensitive to chloramphenicol, and 33 (86.8%) to ciprofloxacin. Resistance to cefoxitin was seen in 7 (18.4%).Conclusion: Results of this study showed that coagulase negative staphylococci were the most common agents causing contact-lens-related microbial keratitis. In addition, gentamycin, chloramphenicol and ciprofloxacin were more suitable than other antibacterial agents against this type of bacteria.
Keywords
Keratitis, Soft contact lens
Coagulase negative staphylococci
Antibacterial resistance
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Journal of Isfahan Medical School
Volume 32, Issue 277 - Serial Number 277
January and February 2014
Pages 273-281

  • Receive Date 10 December 2013
  • Accept Date 06 April 2022