Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Combination Effect of Low-Dose Arsenic Trioxide plus Deferoxamine on Viability and Inhibiting the Proliferation of NB4 Cell Line

Document Type : Original Article (s)

Authors
Kazem Ghaffari 1
Ahmad Kazemi 2
Ali Ghasemi 1
Neda Minayi 1
Abbas Ghotaslou 1
Behnoush Tayebi 3
Mohammad Reza Rezvani 4
1 Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran
2 Professor, Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
3 Department of Hematology, Allied Medical School, Mashhad University of Medical Sciences, Mashhad, Iran
4 Associate Professor, Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Background: Acute promyelocytic leukemia (APL) is one of the most prevalent myeloid lineage leukemia. Currently-available treatment options for these patients are using all trans-retinoic acid (ATRA) and, recently, arsenic trioxide (ATO). All trans-retinoic acid may cause treatment resistance in some cases; and arsenic trioxide, in high doses, has cytotoxic effects. In our study, we determined the ability of deferoxamine (DFO) in reducing the cytotoxic effects of high doses of arsenic on NB4 cell line in the acute promyelocytic leukemia.Methods: The NB4 cell line cultured, expanded and then treated with different doses of deferoxamine (50, 100, and 200 µM) alone, and low dose of arsenic trioxide (0.5 µM) in combination with different doses of deferoxamine (50, 100, 200 µM). Proceeding cells were counted, metabolic activity was measured using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reduction assay and cell viability was determined via trypan blue exclusion assay. The data were analyzed using the Student’s t-test method and the Excel software.Findings: Cells treated with different doses of deferoxamine, alone and in combination with arsenic trioxide, showed decrease in viability and cell activity in a dose- and time-dependent manner. Treatments with combination of 0.5 µM arsenic trioxide and 200 µM deferoxamine showed the maximum effect in decreasing viability and metabolic activity of NB4 cells; and treatments with deferoxamine alone showed the minimum effect in declining viability and metabolic activity.Conclusion: This study shows that deferoxamine has a significant effect on viability of NB4 cell line and also causes decrease in cell activity. It seems that deferoxamine is an appropriate agent for inhibition of tumor cells.
Keywords
Acute promyelocytic leukemia
Arsenic trioxide
Deferoxamine
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Journal of Isfahan Medical School
Volume 32, Issue 288 - Serial Number 288
May and June 2014
Pages 818-827

  • Receive Date 04 January 2014
  • Accept Date 06 April 2022