Journal of Isfahan Medical School

Journal of Isfahan Medical School

Comparing the Perimplantation Thickness of Mouse Uterine Endometrium in Natural Cycle and Those Received Human Menopausal Gonadotropin-Human Chorionic Gonadotropin (HMG–HCG) and Sildenafil Citrate

Document Type : Original Article (s)

Authors
Bahman Rashidi 1
Mohammad Mardani 1
Mostafa Peyvandi-Kariz-Bodagh 2
1 Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 MSc Student, Department of Anatomical Sciences, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Uterine endometrium plays an important role in the implantation process. This layer of the uterus is affected during the menstrual period. Due to the influence of inhibitors and stimulators on uterine endometrium, the aim of this study was to assess the changes in endometrial thickness after injections of sildenafil citrate in super-ovulated mice.Methods: Thirty adult female mice were divided into 3 groups of control, gonadotropin and gonadotropin + sildenafil citrate. In 2 experimental groups, the mice received 7.5 IU of human menopausal gonadotropin (HMG) and later human chorionic gonadotropin (HCG). Then, every two female mice with one male mouse were put in one cage for fertilization. In gonadotropin + sildenafil citrate group, 3 mg/kg sildenafil citrate administrated at 24, 48, and 72 hours after HMG injection. Ninety six hours after HMG injection, the mice in 3 groups were sacrificed and their uterine specimens were prepared for light microscopic studies.Findings: The mean ± SD endometrial thickness was 358.05 ± 13.35 μm in control, 389.84 ± 12.46 μm in gonadotropin and 316.81 ± 12.10 μm in gonadotropin + sildenafil citrate groups. The endometrial thickness in all groups were not significantly deferent from each other (P < 0.05 for all).Conclusion: Using ovarian stimulation, followed by sildenafil citrate injection, would not modify the endometrial thickness of mouse uterus.
Keywords
Endometrium
Endometrial thickness
Sildenafil citrate
Implantation
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Journal of Isfahan Medical School
Volume 32, Issue 300 - Serial Number 300
July and August 2014
Pages 1413-1422

  • Receive Date 25 December 2013
  • Accept Date 06 April 2022