نوع مقاله : مقاله های پژوهشی
1 دانشیار، گروه علوم تشریحی، دانشکدهی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
2 دانشجوی کارشناسی ارشد، گروه علوم تشریحی، دانشکدهی پزشکی و کمیتهی تحقیقات دانشجویی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
عنوان مقاله [English]
Background: Uterine endometrium plays an important role in the implantation process. This layer of the uterus is affected during the menstrual period. Due to the influence of inhibitors and stimulators on uterine endometrium, the aim of this study was to assess the changes in endometrial thickness after injections of sildenafil citrate in super-ovulated mice.Methods: Thirty adult female mice were divided into 3 groups of control, gonadotropin and gonadotropin + sildenafil citrate. In 2 experimental groups, the mice received 7.5 IU of human menopausal gonadotropin (HMG) and later human chorionic gonadotropin (HCG). Then, every two female mice with one male mouse were put in one cage for fertilization. In gonadotropin + sildenafil citrate group, 3 mg/kg sildenafil citrate administrated at 24, 48, and 72 hours after HMG injection. Ninety six hours after HMG injection, the mice in 3 groups were sacrificed and their uterine specimens were prepared for light microscopic studies.Findings: The mean ± SD endometrial thickness was 358.05 ± 13.35 μm in control, 389.84 ± 12.46 μm in gonadotropin and 316.81 ± 12.10 μm in gonadotropin + sildenafil citrate groups. The endometrial thickness in all groups were not significantly deferent from each other (P < 0.05 for all).Conclusion: Using ovarian stimulation, followed by sildenafil citrate injection, would not modify the endometrial thickness of mouse uterus.