تهیه‌ی کونژوگه‌ی پلی‌ساکارید کپسولی نایسریا مننژیتیدیس سروتایپ A با پروتئین ریکامبیننت هپاتیت B به عنوان یک ایمونوژن (تهیه‌ی ایمونوژن از مننژیت و هپاتیت B)

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 کارشناس ارشد، گروه میکروبیولوژی، دانشکده‌ی علوم، دانشگاه آزاد اسلامی واحد کرج، کرج، ایران

2 دانشیار، بخش تهیه واکسن‌های باکتریایی و آنتی‌ژن انستیتو پاستور، مجتمع تولیدی تحقیقاتی انستیتو پاستور ایران، تهران، ایران

3 استاد، گروه میکروبیولوژی، دانشکده‌ی علوم، دانشگاه آزاد اسلامی واحد کرج، کرج، ایران

چکیده

مقدمه: پلی‌ساکارید کپسولی نایسریا مننژیتیدیس سروتایپ A (Neisseria meningitidis capsule یا NMA-CPS) واکسن خوبی برای بیماری مننژیت محسوب می‌شود؛ اما به دلیل این ‌که پاسخ ایمنی نسبت به این پلی‌ساکارید غیر وابسته به سلول T است و سلول خاطره در پاسخ ایمنی ایجاد نمی‌گردد، دوز یادآور (Booster dose) آن تأثیری در بالا بردن سطح ایمنی ندارد. در مطالعه‌ی حاضر، پلی‌ساکارید NMA-CPS به طور کووالان به پروتئین ریکامبیننت هپاتیت B (Recombinant hepatitis B surface antigen یا rHbsAg) متصل گردید.روش‌ها: HbsAg با روش آمیداسیون برای ایجاد یک ایمونوژن دو ظرفیتی مفید و مؤثر، به NMA-CPS کونژوگه شد. کونژوگه و پلی‌ساکارید NMA-CPS به تنهایی به تعدادی خرگوش سفید آزمایشگاهی در سه نوبت به فاصله‌ی 15 روز تزریق گردید و سپس خون‌گیری و جمع‌آوری سرم و بررسی توان باکتری‌کشی سرم با استفاده از روش سرم باکتریسیدال (Serum bactericidal assay یا SBA) صورت گرفت.یافته‌ها: تزریق اول کونژوگه و پلی‌ساکارید تنها، تیتر باکتری‌کشی خوبی را القا نمودند؛ اما کونژوگه در تزریق دوم، تیتر بالاتری را نسبت به پلی‌ساکارید تنها و تزریق اول کونژوگه ایجاد کرد.نتیجه‌گیری: نتایج نشان دهنده‌ی ایجاد سلول‌های خاطره و تأثیر دوز یادآور در بالا بردن سطح ایمنی می‌‌باشد؛ در حالی که تیتر باکتری‌کشی پلی‌ساکارید تنها، در تزریق دوم تغییر کمی داشت؛ این یافته به این دلیل است که پاسخ ایمنی نسبت به این پلی‌ساکارید غیر وابسته به سلول T می‌باشد و سلول خاطره‌ای در پاسخ ایمنی ایجاد نمی‌گردد.

کلیدواژه‌ها

عنوان مقاله [English]

Preparation of ConjugateD Neisseria Meningitidis Type A Capsular Polysaccharide with Recombinant Protein of Hepatitis B as an Immunogen

نویسندگان [English]

  • Mahboobeh Mohamadamini 1
  • Hojat Ahmadi 2
  • Bahman Tabaraie 3
1 Department of Microbiology, School of Science, Karaj Branch, Islamic Azad University, Karaj, Iran
2 Associate Professor, Department of Bacterial Vaccine and Antigen Production, Pasteur Institute of Iran, Tehran, Iran
3 Professor, Department of Microbiology, School of Science, Karaj Branch, Islamic Azad University, Karaj, Iran
چکیده [English]

Background: Polysaccharide vaccines are effective in individuals from about the two years of age but, as they elicit T-cell independent immunity, they are not effective in younger children. In contrast, polysaccharide-protein conjugates are shown to be highly immunogenic in infants and induce T-cell dependent immunity.Methods: In this study, capsular polysaccharide of Neisseria meningitidis type A (NMA-CPS) was attached to recombinant protein of hepatitis B surface antigen (rHbsAg) covalently using amidation method. Immunization was done, choosing 2 groups of rabbits. Pure NMA-CPS and conjugated molecule were injected to groups 1 and 2, with a 15-day interval, intramuscularly. The bleeding was performed at days 0, 15, 30, 45 and titers of sera were measured via serum bactericidal assay.Findings: Polysaccharide bactericidal titer on days 15, 30 and 45 was almost identical and there was no increase in titer. But, in the first injection of the conjugate, the titer was much more (about twice of purified polysaccharide), and in the second injection, increased.Conclusion: Results display that conjugated molecules cause more immunity than pure capsular polysaccharide, and can stimulate cellular immunity.

کلیدواژه‌ها [English]

  • Neisseria meningitidis type A
  • Conjugate
  • Immunogen
  • Recombinant protein
  • Hepatitis B
  • Serum bactericidal assay

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مجله دانشکده پزشکی اصفهان
دوره 33، شماره 359 - شماره پیاپی 359
آذر و دی 1394
صفحه 1955-1964

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  • تاریخ دریافت: 01 تیر 1394
  • تاریخ بازنگری: 01 اردیبهشت 1403
  • تاریخ پذیرش: 17 فروردین 1401

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