ساخت و ارزیابی سازگاری سلولی و زیست‌تخریب ‌پذیری برون‌تن کامپوزیت 3PGS/CaTiO برای کاربرد به عنوان Conduit عصبی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه مهندسی پزشکی- زیست‌مواد، دانشکده‌ی فن‌آوری‌های نوین پزشکی و کمیته‌‌ی تحقیقات دانشجویی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

2 استادیار، گروه بیومتریال، مهندسی بافت و نانوتکنولوژی پزشکی، دانشکده‌ی فن‌آوری‌های نوین پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: Poly(glycerol sebacate) (PGS) پلیمری زیستی و نوین برای کاربرد‌های زیست‌پزشکی با سازگاری زیستی بالا و خواص مکانیکی نزدیک به بافت‌های نرم بدن و همچنین قابلیت تنظیم خواص در حین ساخت می‌باشد؛ اما این پلیمر، به تخریب زیستی به نسبت سریعی دچار می‌شود که به طور معمول، از نرخ بازسازی اعصاب محیطی بالاتر است و برای کاربرد به عنوان Conduit عصبی نیاز به بهبود خواص تخریبی دارد.روش‌ها: ابتدا، پیش‌پلیمر PGS سنتز و سپس معادل 5 درصد وزنی آن سرامیک کلسیم تیتانات (3CaTiO) که سازگاری زیستی بالایی دارد، به آن اضافه شد و مخلوط حاصل تحت عملیات پخت قرار گرفت تا استحکام آن افزایش یابد. بیوکامپوزیت حاصل، مورد تصویربرداری میکروسکوپ الکترونی روبشی، طیف‌سنجی تبدیل Fourier فروسرخ (FTIR یا Fourier transform infrared spectroscopy)، آزمون تخریب زیستی برون‌تن و ارزیابی سمیت سلولی با سلول 929L قرار گرفت.یافته‌ها: در نهایت، کامپوزیتی با توزیع ذرات بالا به دست آمد که آزمون تبدیل Fourier فروسرخ از ایجاد پیوندی شمیایی بین دو پیش‌ساز آن حکایت داشت. زمان تخریب زیستی برون‌تن، 23 درصد کاهش در افت وزن دوره‌ی 60 روزه‌ی تخریب کامپوزیت نسبت به پلیمر خالص را نشان داد. نتایج کشت سلول، زیستایی بالای 90 درصد پس از 5 روز کشت برای این کامپوزیت نشان داد.نتیجه‌گیری: با توجه به نرخ تخریب زیستی، وزن مولکولی و سازگاری سلولی، می‌توان این کامپوزیت را برای کاربرد Conduit عصبی مورد ارزیابی‌های بیشتری قرار داد.

کلیدواژه‌ها

عنوان مقاله [English]

Fabrication and Evaluation of Cell-Compatibility and in-Vitro Biodegradation of PGS/CaTiO3 Composite for Nerve Conduit Application

نویسندگان [English]

  • Reza Naser 1
  • Anousheh Zargar-Kharazi 2
1 MSc Student, Department of Biomedical Engineering and Biomaterials, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
2 Assistant Professor, Department of Biomaterials, Tissue Engineering and Medical Nanotechnology, School of Advanced Medical Technology, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: Poly(glycerol sebacate) (PGS) is a novel biological polymer for biomedical application with high biocompatibility, mechanical properties near to soft tissues of body and a adaptability of properties during synthesis. But, this polymer tends to undergo rather rapid biodegradation which is usually faster than peripheral nerve regeneration and needs optimization of degradability properties for using as a nerve conduit.Methods: First, PGS pre-polymer was synthesized and then, equal to 5% of its weight, calcium titanate ceramic, which is highly biocompatible, was added to it and the acquired mixture was exposed to heat in vacuum oven to increase its strength. The obtained biocomposite came under scanning electron microscopy (SEM) image, Fourier transform infrared (FTIR) spectroscopy, in-vitro biodegradation and cytotoxicity evaluation.Findings: A composite with high particle distribution was obtained which represented a chemical bond between its two precursors. The in-vitro degradation time showed 23% reduction in overall weight loss for the composite in comparison to pure PGS over a period of 60 days degradation. The cell culture showed more than 90% of viability after 5 days of culture for the composite.Conclusion: Regarding to biodegradation rate, molecular mass and high cytocompatibility, this composite is encouraging enough to merit further investigation for nerve conduit application.

کلیدواژه‌ها [English]

  • Poly(glycerol sebacate)
  • Biocomposite
  • Calcium titanate

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مجله دانشکده پزشکی اصفهان
دوره 33، شماره 361 - شماره پیاپی 361
بهمن و اسفند 1394
صفحه 2084-2091

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سابقه مقاله

  • تاریخ دریافت: 15 مرداد 1394
  • تاریخ بازنگری: 08 اردیبهشت 1403
  • تاریخ پذیرش: 17 فروردین 1401

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