بررسی اثرات مواجهه‌ی پیش از تولد با نانوذرات نقره بر تکامل برخی اندام‌های داخلی موش: هیستومورفومتری کبد زاده‌ها

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشیار، گروه فیزیولوژی، دانشکده‌ی دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

2 استاد، گروه بافت‌شناسی، دانشکده‌ی دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

3 کارشناس ارشد، گروه فیزیولوژی، دانشکده‌ی دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

4 دانشجوی دکتری، گروه بافت‌شناسی، دانشکده‌ی دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

5 دانشجوی دکتری، گروه علوم تشریح، دانشکده‌ی دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران

چکیده

مقدمه: با توجه به کاربرد گسترده‌ی نانوذرات نقره در حوزه‌های مختلف زیستی و افزایش مواجهه با آن‌ها، هدف از این مطالعه، بررسی اثرات تزریق زیر جلدی این نانوذرات در طی دوران بارداری بر تکامل برخی اندام‌های داخلی و هیستومورفومتری کبد زاده‌های موش بود.روش‌ها: 15 سر موش ماده‌ی باردار نژاد NMRI در 3 گروه‌، هر 3 روز یک بار تا زمان زایمان، به صورت زیر جلدی نانوذرات نقره را به ترتیب در مقادیر صفر (شاهد)، 2/0 و 2 میلی‌گرم بر کیلوگرم وزن بدن دریافت نمودند. پس از آسان‌کشی، احشای داخلی پنج زاده‌ی نر و پنج زاده‌ی ماده‌ی 28 روزه از هر گروه، استخراج و توزین شد. همچنین، از کبد مقاطع بافتی به ‌منظور بررسی هیستومورفومتری تهیه و با توجه به تیمار و جنسیت واکاوی گردید.یافته‌ها: در مطالعه‌ی حاضر، وزن بدن زاده‌ها اختلاف معنی‌داری نشان نداد (05/0 < P). وزن نسبی طحال در گروه 2 میلی‌گرم بر کیلوگرم نانوذره نسبت به گروه شاهد کمتر بود (01/0 > P). هر چند وزن نسبی کبد در بین تیمارها اختلاف معنی‌داری نداشت (05/0 < P)، اما تأثیر نانوذرات نقره بر وزن کبد، تحت تأثیر جنسیت قرار گرفت (05/0 > P)؛ به ‌گونه‌ای که فقط در زاده‌های نر باعث افزایش وزن کبد (05/0 > P) شد. در گروه‌های تیمار شده با نانوذرات نقره بدون وابستگی به جنسیت، تعداد هپاتوسیت‌ها کمتر و اندازه‌ی آن‌ها بزرگ‌تر از گروه شاهد بود (01/0 > P).نتیجه‌گیری: هر چند مواجهه‌ی موش‌های باردار با نانوذرات نقره بر رشد زاده‌های آن‌ها اثری نداشت، اما باعث افزایش وزن نسبی کبد و تغییرات ساختاری کبد شد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluating the Effects of Prenatal Exposure to Silver Nanoparticles on Development of Some Internal Organs in Mice: Liver Histomorphometry of Offspring

نویسندگان [English]

  • Seyed Reza Fatemi-Tabatabaei 1
  • Naim Erfani-Majd 2
  • Shahab Ghaderi 3
  • Masome Rashno 3
  • Khodabakhsh Rashidi 4
  • Mohammad Mehdi Shamsi 5
  • Mohammad Bahrami-Tapehebur 5
1 Associate Professor, Department of Physiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2 Professor, Department of Histology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3 Department of Physiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4 PhD Student, Department of Histology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
5 PhD Student, Department of Anatomical Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده [English]

Background: Silver nanoparticles (AgNPs) are widely consumed in different fields of biology and exposure to them have increased. This study was designed to evaluate the effects of subcutaneous administration of silver nanoparticles during pregnancy on development of some internal organs and liver histomorphometry of mouse offspring.Methods: Fifteen pregnant NMRI female mice in three groups were subcutaneously administered every three days until delivery with 0 (control), 0.2, and 2 mg/kg of body weight (BW) of silver nanoparticles. Internal organs of five male and five female offspring from each groups were weighed following anesthesia on 28th day offspring. Histological liver sections were also prepared to assess histomorphometry of liver. Finally, results were analyzed according to the treatment and sex.Findings: There were no significant differences in offspring body weights (P > 0.05). Partial weight of spleen were lower in the group received 2 mg/kg nanoparticles compared to the control group (P < 0.01). Although liver partial weight were not significantly different between the groups (P > 0.05), the effect of silver nanoparticles on liver weight was influenced by the sex (P < 0.05), in a way that only the liver weight of male mice were increased (P < 0.05). Number of hepatocytes decreased and their size increased sex-independently in silver-nanoparticles-treated groups compared to control group (P < 0.01).Conclusion: Although exposure of pregnant mice to silver nanoparticles has no effect on the growth of their offspring, it leads to increased liver weight and liver structural changes.

کلیدواژه‌ها [English]

  • Silver nanoparticles
  • Liver histomorphometry
  • Offspring

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مجله دانشکده پزشکی اصفهان
دوره 33، شماره 364 - شماره پیاپی 364
بهمن و اسفند 1394
صفحه 2247-2255

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سابقه مقاله

  • تاریخ دریافت: 18 شهریور 1394
  • تاریخ بازنگری: 30 فروردین 1403
  • تاریخ پذیرش: 17 فروردین 1401

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