تأثیر تمرین هوازی و مصرف امگا 3 بر سطح عامل رشد عصبی هیپوکامپ موش‌های نر سالم و مبتلا به بیماری آلزایمر شده با هوموسیستئین

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه تربیت بدنی، دانشکده ی تربیت بدنی و علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران

2 دانشیار، گروه تربیت بدنی، دانشکده ی تربیت بدنی و علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران

3 استادیار، گروه تربیت بدنی، دانشکده ی تربیت بدنی و علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران

چکیده

مقدمه: مغز، اندامی با سازش پذیری بالا در پاسخ‌های مورفولوژیک، متابولیک و عملکردی به ورزش و تغذیه است. هدف از انجام پژوهش حاضر، بررسی تأثیر مصرف امگا 3 و تمرین هوازی بر سطح عامل رشد عصبی (NGF یا Nerve growth factor) هیپوکامپ در موش‌های نر سالم و مبتلا به بیماری آلزایمر شده با هوموسیستئین بود.روش‌ها: 90 سر موش صحرایی نر نژاد Wistar (با سن 12 هفته و میانگین وزن 60/12 ± 99/219 گرم)، به 9 گروه تقسیم شدند. برای القای بیماری آلزایمر، از تزریق هوموسیستئین با دز 6/0 مولار به درون بطن مغز موش‌ها استفاده شد. تمرین هوازی روی نوار گردان ( 5 روز در هفته، با شدت 55-50 درصد حداکثر اکسیژن مصرفی) و مکمل (روزانه 800 میلی‌گرم به ازای هر کیلوگرم وزن بدن) به مدت 8 هفته اعمال گردید. 72 ساعت پس از آخرین جلسه‌ی مداخله، موش‌ها بیهوش و جراحی شدند و بافت هیپوکامپ جدا شد. داده‌ها با استفاده از آزمون‌ One-way ANOVA و آزمون تعقیبی Tukey تحلیل گردید (050/0 > P).یافته‌ها: در آزمودنی‌های سالم، مصرف امگا 3 باعث افزایش معنی‌دار سطح NGF شد (001/0 = P). انجام تمرین (990/0 = P) و همراه نمودن تمرین و امگا 3 (210/0 = P)، تغییر معنی‌داری در سطح NGF ایجاد نکرد. در آزمودنی‌های مبتلا به بیماری آلزایمر، تمرین هوازی باعث افزایش معنی‌دار سطح NGF گردید (020/0 = P) و مصرف امگا 3 (930/0 = P) و همراه نمودن آن با تمرین (220/0 = P)، تغییر معنی‌داری در سطح NGF ایجاد نکرد.نتیجه‌گیری: به نظر می‌رسد انجام تمرین و مصرف امگا 3 به عنوان مداخلات درمانی در راهبردهای پیش‌گیری و درمان بیماری آلزایمر، به صورت‌های مختلفی تأثیر می‌گذارد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Aerobic Training and Omega-3 Intake on Nerve Growth Factor in the Hippocampus of Healthy Male Rats and Rats with Homocysteine Induced Alzheimer's Model

نویسندگان [English]

  • Raziyeh Norouzi-Kakhki 1
  • Marziyeh Saghebjoo 2
  • Ali Seghatoleslami 3
1 MSc Student, Department of Physical Education, School of Physical Education and Sport Sciences, University of Birjand, Birjand, Iran
2 Associate Professor, Department of Physical Education, School of Physical Education and Sport Sciences, University of Birjand, Birjand, Iran
3 Assistant Professor, Department of Physical Education, School of Physical Education and Sport Sciences, University of Birjand, Birjand, Iran
چکیده [English]

Background: The brain is organ that has high adaptability in response to morphological, metabolic and functional to exercise and nutrition. The aim of this study was to examine the effect of omega-3 intake and aerobic training on nerve growth factor (NGF) in the hippocampus of healthy male rats and rats with homocysteine induced Alzheimer's model.Methods: Ninety male Wistar rats (12 weeks old and mean weight 219.99 ± 12.60 g), were divided into 9 groups. To induce Alzheimer's disease, homocysteine was infused into the rats cerebroventricule at a dose of 0.6M were used. Aerobic training on a treadmill (5 days per week, with 50 to 55% of maximal oxygen consumption) were carried out and supplemented groups during the 8 weeks, daily 800 mg per kg body weight were administered omega-3 supplements. 72 hours after the last intervention session, the rats anesthetized, and surgically removed the hippocampus tissue. Data analysis using one way ANOVA and Tukey tests were performed (P < 0.05).Findings: In healthy subjects, intake of omega-3 caused a significant increase in NGF level (P = 0.001). Exercise training and absence of omega-3 along with exercise training, had no significant effect on NGF levels (P = 0.990 and P = 0.210 respectively). In subjects with Alzheimer's disease, aerobic training caused a significant increase in NGF levels (P = 0.020), and omega-3 intake with its combination with exercise training, had no significant change in the level of NGF (P = 0.930 and P = 0.220 respectively).Conclusion: It seems that exercise training and omega-3 intake in the strategy of prevention and treatment of Alzheimer's disease can affect in many different forms.

کلیدواژه‌ها [English]

  • Aerobic training
  • Omega 3
  • Alzheimer
  • Nerve growth factor

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مجله دانشکده پزشکی اصفهان
دوره 34، شماره 379 - شماره پیاپی 379
خرداد و تیر 1395
صفحه 392-400

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سابقه مقاله

  • تاریخ دریافت: 23 دی 1394
  • تاریخ بازنگری: 05 اردیبهشت 1403
  • تاریخ پذیرش: 17 فروردین 1401

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