Journal of Isfahan Medical School

Journal of Isfahan Medical School

Identification of Candida Species Isolated from Candiduria Patients Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism in Isfahan, Iran

Document Type : Original Article (s)

Authors
Parvin Dehghan 1
Mohammad Bahmaei 2
Rasoul Mohammadi 1
Javaher Chabavizadeh 1
Behzad Mahaki 3
1 Assistant Professor, Department of Mycology and Parasitology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 MSc Student, Department of Mycology and Parasitology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
3 Assistant Professor, Department of Biostatistics, School of Health, Isfahan University of Medical Sciences, Isfahan Iran
Abstract
Background: Disseminated candidiasis, diabetes mellitus, pregnancy, and consumption of widespread antibiotics are predisposing factors of urinary tract infections due to Candida species. The prevalence of non-albicans species is increasing. The aim of the present study is to identify Candida spp. isolated from candiduria patients in Isfahan, Iran using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Methods: The genomic DNA was extracted by fast technology for analysis-card (FTA-card). Polymerase chain reaction (PCR) was used to amplify ITS1-5.8s-ITS2 region then PCR products were digested with MspI restriction enzyme. PCR and RFLP products were revealed on 1.5 and 2% agarose gel electrophoresis, respectively.Findings: Out of 3200 urine samples 80 samples with more than 104 CFU/ml were involved in this study. Patients including 4 (5%) were males and 76 patients (95%) were females. Predisposing factors were diabetes (47.5%), urinary tract infection (38.8%) and kidney stone (5.0%). The colony count of 46.3% of the urine samples were with 50×103-99×103 CFU/ml. Candida glabrata was the most prevalent species among isolates (41.3%).Conclusion: Increasing of non-albicans Candida species and drug resistance of isolates are main challenges of Candida infection treatment particularly in patients with diabetes and urinary infections. Identification of Candida species is inevitable for better management of infection.
Keywords
Identification
Candiduria
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)
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Journal of Isfahan Medical School
Volume 34, Issue 381 - Serial Number 381
March and April 2016
Pages 484-490

  • Receive Date 09 February 2016
  • Accept Date 06 April 2022