ارزیابی آنژیوژنز و ضخامت میومتریوم رحم موش، بلافاصله قبل از لانه‌گزینی در چرخه‌ی طبیعی، همراه با تحریک تخمک‌گذاری و مصرف سیلدنافیل سیترات

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشیار، گروه علوم تشریحی و زیست‌شناسی مولکولی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

2 دانشجوی پزشکی، کمیته‌ی تحقیقات دانشجویی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

3 دانشجوی دکتری تخصصی، گروه علوم تشریحی و زیست‌شناسی مولکولی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: انقباضات میومتر رحمی، نقش کلیدی در شکست یا موفقیت لانه‌گزینی رویان بازی می‌کند. افزون بر آن، آنژیوژنز کافی در میومتریوم، یک رویداد مهم برای تأمین خون اندومتریوم می‌باشد. به تازگی، مطالعات زیادی به منظور بررسی اثرات سیلدنافیل سیترات بر روی باروری زنان انجام شده است. این مطالعه، با هدف بررسی تغییرات ضخامت میومتریوم و آنژیوژنز وابسته به آن به دنبال تجویز سیلدنافیل سیترات در موش‌هایی که تحریک تخمدانی شده‌اند انجام شد.روش‌ها: 30 سر موش بالغ ماده و 10 موش بالغ نر خریداری شدند. موش‌های ماده به سه گروه 10تایی شاهد (بدون هیچ گونه مداخله)، گونادوتروپین (دریافت کننده‌ی Human menopausal gonadotropin یا HMG و Human chorionic gonadotropin یا HCG) و گونادوتروپین + سیلدنافیل سیترات (دریافت کننده‌ی HMG، HCG و سیلدنافیل سیترات) تقسیم شدند. بعد از جفت‌گیری، حیوانات بیهوش و رحم آن‌ها به طور سریع برای اهداف بافت‌شناسی و ایمونوهیستوشیمی برای سلول‌های Cluster of differentiation 31 (CD31) خارج شد.یافته‌ها: در گروه سیلدنافیل سیترات + گونادوتروپین، ضخامت میومتریوم به طور معنی‌داری بیشتر از گروه‌های گونادوتروپین و شاهد بود (05/0 > P). نتایج ایمونوهیستوشیمی برای سلول‌های CD31 در میوتریوم نشان داد که تجویز سیلدنافیل سیترات هیچ تغییر معنی‌دار مرتبط با آنژیوژنز را در میومتریوم القا نمی‌کند (05/0 < P).نتیجه‌گیری: تجویز سیلدنافیل سیترات، هیچ تغییر مشهودی را در آنژیوژنز میومتریوم ایجاد نمی‌کند، اما سبب افزایش ضخامت آن می‌شود. با این وجود، برای ابراز تصمیم دقیق در مورد نقش سیلدنافیل سیترات بر روی پارامترهای اشاره شده، بررسی‌های دقیق مولکولی و افزایش مدت زمان مصرف دارو ضروری می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Survey of Angiogenesis and Myometrial Thickness of Preimplantation Mouse Uterine in Natural Cycle in those Received Human Menopausal Gonadotropin-Human Chorionic Gonadotropin (HMG–HCG) and Sildenafil Citrate

نویسندگان [English]

  • Gholamreza Dashti 1
  • Ariubarzan Farhadi 2
  • Mohammad Javad Saeedi-Borujeni 3
  • Bahman Rashidi 1
1 Associate Professor, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Student of Medicine, Student Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 PhD Candidate, Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: Uterus myometrium contractions play crucial role in break or successful embryo implantation. Furthermore, efficient myometrial angiogenesis is important event for supplying endometrium blood. Recently, many studies were performed for investigating the effects of sildenafil citrate (SC) on female fertility. This study aimed was to assess the changes in myometrial thickness and associated angiogenesis after injections of sildenafil citrate in superovulated mice.Methods: Thirty adult female mice and ten adult male mice were purchased. The female mice were divided into three equal groups of control without any intervention, gonadotropin receiving human menopausal gonadotropin (HMG) and human chorionic gonadotropin (HCG), and gonadotropin and sildenafil citrate receiving human menopausal gonadotropin, and human chorionic gonadotropin and sildenafil citrate administration. After mating, animals were deeply anesthetized and the uterus was rapidly removed for histology and immunohistochemistry process to identify cluster of differentiation 31 (CD31).Findings: In gonadotropin + sildenafil citrate group, the thickness of myometrium was significantly thicker than control and gonadotropin groups (P < 0.05 for both). The results of immunohistochemistry survey for myometrium CD31 demonstrated that administrated sildenafil citrate did not induce any angiogenesis associated alteration in gonadotropin + sildenafil citrate group (P > 0.05).Conclusion: It may finally conclude that administration of sildenafil citrate does not cause remarkable alterations in myometrial angiogenesis; but increases the thickness of myometrium. Although for realistic decision about the sildenafil citrate effects on aforementioned parameters, more molecular investigations and longer drug consumption periods are necessary.

کلیدواژه‌ها [English]

  • Sildenafil citrate
  • Myometrium
  • Angiogenesis
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