Journal of Isfahan Medical School

Journal of Isfahan Medical School

Association between the Length of a CA Dinucleotide Repeat in the c-src Gene and Risk of Prostate Cancer

Document Type : Original Article (s)

Authors
Mohammad Amin Ezhehi 1
Manoochehr Tavassoli 2
Simin Hematti 3
Forouzan Safari 4
1 MSc Student, Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran
2 Professor, Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran
3 Assistant Professor, Department of Radiotherapy, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran
10.22122/jims.v35i454.8171
Abstract
Background: Src is a non-receptor protein tyrosine kinase that has a crucial role in signaling pathways involved in cell division, motility, adhesion, and survival in cells. To date, there has been no report on the association between polymorphism of CA dinucleotide repeat in the c-src gene and risk of prostate cancer. In this study, the association between CA polymorphism in intron 2 of the c-src gene and the risk of prostate cancer was investigated.Methods: A group of 142 patients with prostate cancer and 135 healthy men were selected. Genomic DNA was extracted from white blood cells and desired sequence was amplified via the polymerase chain reaction (PCR). The number of CA repetitions was obtained using polyacrylamide gel electrophoresis and sequencing.Findings: 15 different alleles were identified in 277 subjects; their distribution varied between 13 and 28 repeats. 22 repeat allele was associated with an increased risk of prostate cancer [Odds ratio (OR) = 2.31, P = 0.0005] and men with genotypes 22-CA/20-CA and 22-CA/19-CA were at higher risk of developing prostate cancer (OR = 5.5, P = 0.0001).Conclusion: Our findings demonstrate that men with 22 CA repeat are at a significantly higher risk of prostate cancer. In conclusion, our result suggests a potential role for this microsatellite as a predictive marker of prostate cancer risk in Iranian men.
Keywords
Prostate cancer
c-src genes, Polymorphism
Microsatellites
  1. Araujo J, Logothetis C. Targeting Src signaling in metastatic bone disease. Int J Cancer 2009; 124(1): 1-6.
  2. Reynolds AB, Roczniak-Ferguson A. Emerging roles for p120-catenin in cell adhesion and cancer. Oncogene 2004; 23(48): 7947-56.
  3. Patel A, Sabbineni H, Clarke A, Somanath PR. Novel roles of Src in cancer cell epithelial-to-mesenchymal transition, vascular permeability, microinvasion and metastasis. Life Sci 2016; 157: 52-61.
  4. Varkaris A, Katsiampoura AD, Araujo JC, Gallick GE, Corn PG. Src signaling pathways in prostate cancer. Cancer Metastasis Rev 2014; 33(2-3): 595-606.
  5. Tatarov O, Mitchell TJ, Seywright M, Leung HY, Brunton VG, Edwards J. SRC family kinase activity is up-regulated in hormone-refractory prostate cancer. Clin Cancer Res 2009; 15(10): 3540-9.
  6. Usdin K. The biological effects of simple tandem repeats: Lessons from the repeat expansion diseases. Genome Res 2008; 18(7): 1011-9.
  7. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988; 16(3): 1215.
  8. Cai H, Babic I, Wei X, Huang J, Witte ON. Invasive prostate carcinoma driven by c-Src and androgen receptor synergy. Cancer Res 2011; 71(3): 862-72.
  9. Zhang W, Gordon M, Yun J, Press O, Yang D, Groshen S, et al. Association between dinucleotide repeat(CA) polymorphism of nuclear factor kappa-bata(NF-KB) and local recurrence of stage II/III rectal cancer patients treated with chemoradiation. Int J Radiat Oncol Biol Phys 2004; 60(1): S423.
  10. Zhang W, He L, Liu W, Sun C, Ratain MJ. Exploring the relationship between polymorphic (TG/CA)n repeats in intron 1 regions and gene expression. Hum Genomics 2009; 3(3): 236-45.
  11. Hafezi G, Tavssoli M, Hemmati S, Safari F. The association between CA polymorphism in src gene and risk of breast cancer. J Isfahan Med Sch 2017; 35(419): 87-92. [In Persian].
Journal of Isfahan Medical School
Volume 35, Issue 454 - Serial Number 454
September and October 2018
Pages 1587-1592

  • Receive Date 04 May 2017
  • Accept Date 06 April 2022