بررسی ارتباط پلی‌مورفیسم در ژن Xrcc3 با سرطان‌ کولورکتال: یک مطالعه‌ی مورد- شاهدی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشیار، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه اصفهان، اصفهان، ایران

2 دانشجوی دکتری، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه اصفهان، اصفهان، ایران

3 دانشجوی کارشناسی ارشد، گروه زیست‌شناسی، دانشکده‌ی علوم، دانشگاه اصفهان، اصفهان، ایران

چکیده

مقدمه: سرطان‌ کولورکتال، سومین سرطان منجر به مرگ در کشورهای ‌غربی محسوب می‌شود. سن بالا، چاقی، بی‌تحرکی، رژیم غذایی نامناسب و تغییرات ژنتیکی، از‌ جمله عوامل خطر بروز این سرطان به‌ شمار می‌رود. بررسی ارتباط تغییرات ژنتیکی با سرطان ‌کولورکتال، زمینه‌ی بسیاری ‌از تحقیقات اخیر بوده و پلی‌مورفیسم M241T در ژن Xrcc3 مورد توجه قرار گرفته ‌است.روش‌ها: در این پژوهش مورد- شاهدی، 90 بیمار مبتلا به سرطان کولورکتال و 83 فرد سالم مورد مطالعه قرار گرفتند. DNA ژنومی از خون محیطی افراد استخراج و توزیع ژنوتیپی در محل پلی‌مورفیسم مذکور بررسی گردید. منطقه‌ی مورد ‌نظر با استفاده از طراحی پرایمر و تکنیک Polymerase chain reaction (PCR) تشدید ژنی شد و ژنوتیپ نمونه‌ها با روش PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) تعیین گردید.یافته‌ها: یافته‌ها حاکی از ارتباط آلل C از پلی‌مورفیسم M241T با ابتلا به سرطان‌ کولورکتال بود. همچنین، ارتباط معنی‌داری بین افزایش سن و سابقه‌ی فامیلی با بروز سرطان مشاهده ‌شد. از طرف دیگر، مصرف سیگار با سرطان ‌کولون رابطه‌ای را نشان نداد، اما نقش مؤثری در بروز و متاستاز سرطان ‌رکتوم داشت.نتیجه‌گیری: ارتباط پلی‌مورفیسم M241T با افزایش خطر بروز سرطان‌ کولورکتال در‌ کنار سابقه‌ی‌ فامیلی، سن بالا و مصرف سیگار، به عنوان عوامل خطر ابتلا به سرطان‌ کولورکتال گزارش گردید. از این‌رو، نتایج به دست آمده از پژوهش حاضر، ضرورت بررسی و انجام تحقیقات گسترده‌تر در مطالعات بعدی را مشخص نمود

کلیدواژه‌ها

عنوان مقاله [English]

The Association between Polymorphism of Xrcc3 Gene and Colorectal Cancer: A Case-Control Study

نویسندگان [English]

  • Majid Motovali-Bashi 1
  • Halimeh Rezaei 2
  • Jafar Maleki 3
  • Sheyda Khalilian 3
1 Associate Professor, Department of Biology, School of Science, University of Isfahan, Isfahan, Iran
2 PhD Student, Department of Biology, School of Science, University of Isfahan, Isfahan, Iran
3 MSc Student, Department of Biology, School of Science, University of Isfahan, Isfahan, Iran
چکیده [English]

Background: Colorectal cancer is the third cancer which results in death in western countries. Some of the risk factors of this cancer are age, inadequate diet, obesity, inactivity, genetic changes, etc. Considering the relationship between genetic changes with colorectal cancer is the subject of recent studies, and one of the subjects which have been focused is T241M polymorphism in Xrcc3 gene.Methods: In this case-control study, 90 patients with colorectal cancer and 83 healthy people were included. Genomic DNA was extracted from peripheral blood, and genotype distribution in the region of polymorphism was studied. By designing of primers, the considered area was amplified and genotyped using polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) method.Findings: We observed the association between T241M polymorphism and colorectal cancer. Besides, there was a meaningful relationship between aging and family history with this cancer. On the other hand, smoking was not associated with colon cancer, but it also showed a significant role in the incidence and metastasis of rectal cancer.Conclusion: The relationship between T241M polymorphism and increased risk of colorectal cancer, along with family history of cancer, old age, and smoking were reported as risk factors for colorectal cancer. Therefore, the results of this research can show the need for further investigation in subsequent studies.

کلیدواژه‌ها [English]

  • Colorectal Cancer
  • Aging
  • Gender
  • Smoking

مراجع

  1. Hadjipetrou A, Anyfantakis D, Galanakis CG, Kastanakis M, Kastanakis S. Colorectal cancer, screening and primary care: A mini literature review. World J Gastroenterol 2017; 23(33): 6049-58.
  2. Khosravi SF, Ayubi E, Khazaei S, Sani M, Mansouri HS, Khazaei S, et al. Geographic distribution of the incidence of colorectal cancer in Iran: A population-based study. Epidemiol Health 2017; 39: e2017020.
  3. Rafiemanesh H, Pakzad R, Abedi M, Kor Y, Moludi J, Towhidi F, et al. Colorectal cancer in Iran: Epidemiology and morphology trends. EXCLI J 2016; 15: 738-44.
  4. van der Beek CM, Dejong CHC, Troost FJ, Masclee AAM, Lenaerts K. Role of short-chain fatty acids in colonic inflammation, carcinogenesis, and mucosal protection and healing. Nutr Rev 2017; 75(4): 286-305.
  5. Vargas AJ, Wertheim BC, Gerner EW, Thomson CA, Rock CL, Thompson PA. Dietary polyamine intake and risk of colorectal adenomatous polyps. Am J Clin Nutr 2012; 96(1): 133-41.
  6. Walsh C. Colorectal polyps: Cancer risk and classification. Gastrointestinal Nursing 2017; 15(5): 26-32.
  7. Govaert KM, Jongen JMJ, Kranenburg O, Borel Rinkes IHM. Surgery-induced tumor growth in (metastatic) colorectal cancer. Surg Oncol 2017; 26(4): 535-43.
  8. Sakita JY, Gasparotto B, Garcia SB, Uyemura SA, Kannen V. A critical discussion on diet, genomic mutations and repair mechanisms in colon carcinogenesis. Toxicol Lett 2017; 265: 106-16.
  9. Shen H, Yang J, Huang Q, Jiang MJ, Tan YN, Fu JF, et al. Different treatment strategies and molecular features between right-sided and left-sided colon cancers. World J Gastroenterol 2015; 21(21): 6470-8.
  10. Namazi A, Abedinzadeh M, Nourbaksh P, Neamatzadeh H. Association between the XRCC3 Thr241Met polymorphism and risk of colorectal cancer: a meta analysis of 5,193 cases and 6,645 controls. Asian Pac J Cancer Prev 2015; 16(6): 2263-8.
  11. Krupa R, Sliwinski T, Wisniewska-Jarosinska M, Chojnacki J, Wasylecka M, Dziki L, et al. Polymorphisms in RAD51, XRCC2 and XRCC3 genes of the homologous recombination repair in colorectal cancer--a case control study. Mol Biol Rep 2011; 38(4): 2849-54.
  12. Zhao Y, Deng X, Wang Z, Wang Q, Liu Y. Genetic polymorphisms of DNA repair genes XRCC1 and XRCC3 and risk of colorectal cancer in Chinese population. Asian Pac J Cancer Prev 2012; 13(2): 665-9.
  13. Alayev A, Salamon RS, Manna S, Schwartz NS, Berman AY, Holz MK. Estrogen induces RAD51C expression and localization to sites of DNA damage. Cell Cycle 2016; 15(23): 3230-9.
  14. Ji RB, Qian YS, Hu AR, Hu YR. DNA repair gene XRCC3 T241M polymorphism and susceptibility to hepatocellular carcinoma in a Chinese population: a meta-analysis. Genet Mol Res 2015; 14(4): 15988-96.
  15. Mucha B, Przybylowska-Sygut K, Dziki AJ, Dziki L, Sygut A, Majsterek I. Association of Thr241Met polymorphism of XRCC3 gene with risk of colorectal cancer in the Polish population. Pol J Pathol 2013; 64(3): 185-90.
  16. Motovali-Bashi M, Maleki J, Hemmati S, Korbekandi H. Corelation of homologous recombination repair system by studying a single-nucleotide polymorphism in xrcc3 gene with initiation and progression of colorectal cancer. J Isfahan Med Sch 2011; 29(137): 518-25. [In Persian].
  17. Al-Shuhaib Mohammed Baqur SA. A universal, rapid, and inexpensive method for genomic DNA isolation from the whole blood of mammals and birds. J Genet 2017; 96(1): 171-6.
  18. He XF, Wei W, Li JL, Shen XL, Ding DP, Wang SL, et al. Association between the XRCC3 T241M polymorphism and risk of cancer: evidence from 157 case-control studies. Gene 2013; 523(1): 10-9.
  19. Surdu S, Fitzgerald EF, Bloom MS, Boscoe FP, Carpenter DO, Haase RF, et al. Polymorphisms in DNA repair genes XRCC1 and XRCC3, occupational exposure to arsenic and sunlight, and the risk of non-melanoma skin cancer in a European case-control study. Environ Res 2014; 134: 382-9.
  20. Figl A, Scherer D, Nagore E, Bermejo JL, Botella-Estrada R, Gast A, et al. Single-nucleotide polymorphisms in DNA-repair genes and cutaneous melanoma. Mutat Res 2010; 702(1): 8-16.
  21. Savina NV, Nikitchenko NV, Kuzhir TD, Rolevich AI, Krasny SA, Goncharova RI. The Cellular Response to Oxidatively Induced DNA Damage and Polymorphism of Some DNA Repair Genes Associated with Clinicopathological Features of Bladder Cancer. Oxid Med Cell Longev 2016; 2016: 5710403.
  22. Shadrina AS, Ermolenko NA, Boyarskikh UA, Sinkina TV, Lazarev AF, Petrova VD, et al. Polymorphisms in DNA repair genes and breast cancer risk in Russian population: a case-control study. Clin Exp Med 2016; 16(1): 21-8.
  23. Ding P, Yang Y, Cheng L, Zhang X, Cheng L, Li C, et al. The relationship between seven common polymorphisms from five DNA repair genes and the risk for breast cancer in northern Chinese women. PLoS One 2014; 9(3): e92083.
  24. Liu HX, Li J, Ye BG. Correlation between gene polymorphisms of CYP1A1, GSTP1, ERCC2, XRCC1, and XRCC3 and susceptibility to lung cancer. Genet Mol Res 2016; 15(4).
  25. Nissar S, Sameer AS, Lone TA, Chowdri NA, Rasool R. XRCC3 Thr241Met gene polymorphism and risk of colorectal cancer in Kashmir: a case control study. Asian Pac J Cancer Prev 2014; 15(22): 9621-5.
  26. Procopciuc LM, Osian G, Iancu M. Colorectal Cancer Carcinogenesis: a Multivariate Genetic Model in a Cohort of Romanian Population. Clin Lab 2017; 63(4): 647-58.
  27. Chen K, Xia G, Zhang C, Sun Y. Correlation between smoking history and molecular pathways in sporadic colorectal cancer: a meta-analysis. Int J Clin Exp Med 2015; 8(3): 3241-57.
  28. Barrow TM, Klett H, Toth R, Bohm J, Gigic B, Habermann N, et al. Smoking is associated with hypermethylation of the APC 1A promoter in colorectal cancer: the ColoCare Study. J Pathol 2017; 243(3): 366-75.
مجله دانشکده پزشکی اصفهان
دوره 36، شماره 475 - شماره پیاپی 475
خرداد و تیر 1397
صفحه 366-371

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سابقه مقاله

  • تاریخ دریافت: 23 فروردین 1397
  • تاریخ بازنگری: 07 اردیبهشت 1403
  • تاریخ پذیرش: 17 فروردین 1401

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