اثرات ژنوتوکسیک احتمالی ناشی از تماس با سم فوزالون در سلول‌های مغز قرمز استخوان موش سوری

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی داروسازی، گروه فارماکولوژی و سم‌شناسی، دانشکده‌ی داروسازی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

2 دانشیار، گروه فارماکولوژی و سم‌شناسی، دانشکده‌ی داروسازی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

3 استادیار، گروه فارماکولوژی و سم‌شناسی، دانشکده‌ی داروسازی و مرکز تحقیقات علوم دارویی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران

چکیده

مقدمه: فوزالون، به عنوان یک آفت‌کش ارگانوفسفره جهت کنترل آفات مورد استفاده قرار می‌گیرد. استرس اکسیداتیو، یکی از مهم‌ترین مکانیسم‌هایی است که فوزالون از طریق آن باعث سمیت می‌شود. مطالعه‌ی حاضر، با هدف ارزیابی اثرات ژنوتوکسیک احتمالی به دنبال تماس با سم فوزالون در سلول‌های مغز قرمز استخوان موش سوری انجام شد.روش‌ها: در این مطالعه، از 60 سر موش سوری سالم نر بالغ (سن 8-6 هفته و وزن تقریبی بین 25-20 گرم) استفاده شد که به 6 گروه تقسیم شدند (10 = n در هر گروه). گروه 1، حامل سم فوزرالون به مدت 5 روز متوالی به صورت گاواژ (گروه شاهد منفی)، گروه 2، داروی سیکلوفسفامید با دز 40 میلی‌گرم/کیلوگرم به صورت داخل صفاقی (شاهد مثبت) و گروه‌های 3، 4، 5 و 6 فوزالون را در دزهای 40، 20، 12 و 6 میلی‌گرم/کیلوگرم وزن بدن در 5 روز متوالی به صورت گاواژ دریافت کردند. پس از جداسازی مغز استخوان و لنفوسیت‌ها از موش‌های سوری، آزمون Comet جهت ارزیابی اثرات ژنوتوکسیک به کار گرفته شد.یافته‌ها: میزان Tail length در گروه‌های دریافت کننده‌ی فوزالون به مدت 1 و 5 روز در دزهای 12، 20 و 40 میلی‌گرم/کیلوگرم با گروه شاهد منفی (حامل سم فوزالون) تفاوت معنی‌داری داشت (050/0 > P). همچنین، عامل زمان نیز در افزایش این تخریب مؤثر است؛ به گونه‌ای که در بررسی 5 روزه نسبت به 1 روزه تخریب DNA بیشتری مشاهده شد.نتیجه‌گیری: در این مطالعه، اثرات ژنوتوکسیک وابسته به دز و زمان با سم فوزالون مشاهده شد. از این رو، پیشنهاد می‌گردد توجه جدی در محدودیت استفاده از این ترکیب به عنوان آفت‌کش در عرصه‌ی کشاورزی صورت پذیرد.

کلیدواژه‌ها


عنوان مقاله [English]

The Probable Genotoxic Effects of Phosalone Exposure in Mice Bone Marrow Cells

نویسندگان [English]

  • Zohreh Khodabandeh 1
  • Mahmoud Etebari 2
  • Mehdi Aliomrani 3
1 Pharm D Student, Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2 Associate Professor, Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
3 Assistant Professor, Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده [English]

Background: Phosalone is used as a pesticide to control pests. Oxidative stress is one of the most important mechanisms by which Phosalone causes toxicity. The aim of this study was to evaluate the possible genotoxic effects following exposure to Phosalone in red bone marrow cells of mice.Methods: In this study, 60 Syrian male mice [with 6 to 8 weeks of age and body weight (BW) of 20-25 g] were divided to 8 equal groups. Group 1 received Phosalone career by gavage for 5 days (negative control), group 2 received cyclophosphamide with the dose of 40 mg/kg by gavage for 5 days (positive control), and groups 3, 4, 5, and 6 received Phosalone at doses of 6, 12, 20, and 40 mg/kg BW for 5 consecutive days by gavage. After isolating bone marrow and lymphocytes from mice, comet test was performed to evaluate genotoxic effects 1 and 5 days following exposure to Phosalone.Findings: Tail length in the groups receiving Phosalone for 1 day and 5 days at doses of 12, 20, and 40 mg/kg BW was significantly different from the negative control group (P < 0.050). The time factor was also effective in increasing this degradation; as in 5-day assessment more DNA degradation was observed than 1-day assessment.Conclusion: In this study, dose- and time-dependent genotoxic effects with Phosalone were observed. It also highlights the need for serious attention to limiting the use of this compound as a pesticide in agriculture.

کلیدواژه‌ها [English]

  • Organophosphates
  • Phosalone
  • Comet assay
  • Cyclophosphamide
  • Zolone
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