وجود هم‌زمان سایتومگالوویروس در سرم بیماران مبتلا به لوسمی

نوع مقاله : مقاله های پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه میکروبیولوژی، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران

2 استاد، گروه میکروبیولوژی، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران

3 استادیار، گروه میکروبیولوژی، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

مقدمه: لوسمی با تخریب سلول‌های خون‌ساز مغز استخوان شروع می‌شود و با آشفتگی در خون (تکثیر گلبول‌های نابالغ، اختلال در هموستازی، درجاتی از التهاب، جهش، فقدان ایمنی، فقر آنتی‌اکسیدان‌ها و عوامل عفونی) بروز می‌نماید. سایتومگالوویروس (Cytomegalovirus یا CMV) به عنوان بزرگ‌ترین عضو خانواده‌ی Herpesviridae می‌تواند عفونت نهفته برای تمام عمر ایجاد کند. از این رو، با توجه به آمار بالای ابتلا به لوسمی در ایران، مطالعه‌ی حاضر با هدف ارزیابی فراوانی CMV در موارد ابتلا به لوسمی به روش Polymerase chain reaction (PCR) انجام شد.روش‌ها: 100 نمونه سرم خون افراد مبتلا به لوسمی و 100 نمونه‌ی شاهد سالم، از بیمارستان‌های تهران جمع‌آوری و استخراج DNA به روش فنل‌کلروفرم انجام شد. آزمایش PCR تشخیص CMV بهینه و از جهت ویژگی و حد تشخیص (Limit of detection یا LOD) بررسی گردید. آزمایش بهینه بر روی همه‌ی نمونه‌های مورد و شاهد در کنار شاهد مثبت و منفی انجام و محصول PCR به روش آگارز ژل الکتروفورز شناسایی شد.یافته‌ها: آزمایش PCR بهینه و محصول 257 جفت‌باز در آگارز ژل الکتروفورز مشاهده شد. در آزمایش ویژگی، هیچ محصولی به غیر از CMV مشاهده نشد. حد تشخیص Copy/reaction 100 به دست آمد. تمامی نمونه‌های شاهد سالم منفی و 33 درصد از نمونه‌های بیماران، مثبت گردید.نتیجه‌گیری: نتایج این مطالعه نشان داد که CMV می‌تواند از عوامل عفونی و تأثیرگذار در لوسمی در نظر گرفته شود.

کلیدواژه‌ها

عنوان مقاله [English]

Coexistence of Cytomegalovirus in Serum of Patients Suffering from Leukemia

نویسندگان [English]

  • Taraneh Rahenow 1
  • Mohammad Hassan Shahhosseiny 2
  • Taher Mohammadian 3
  • Atousa Ferdosi 3
1 PhD Student, Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University. Tehran,
2 Professor, Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University. Tehran, Iran
3 Assistant Professor, Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University. Tehran, Iran
چکیده [English]

Background: Leukemia begins with the destruction of hematopoietic bone marrow cells, and appears with the turmoil in the blood (immature blood cell proliferation, homeostasis disorder, degrees of inflammation, mutation, immunodeficiency, antioxidant deficiency, and infectious agents). Cytomegalovirus (CMV) as the largest member of the herpes family can cause latent infections for lifetime. Due to its high numbers in Iran, we decided to measure the level of CMV in leukemia by polymerase chain reaction (PCR) method, to quantify its prevalence.Methods: 100 blood serum samples of people with leukemia and 100 healthy controls were collected from hospitals in Tehran City, Iran, and DNA was extracted by phenol-chloroform method. PCR test of CMV was optimized and evaluated for specificity and limit of detection (LOD). Optimized test was performed on all patient and healthy samples along with positive and negative controls. The PCR product was identified by agarose gel electrophoresis.Findings: PCR test was optimized, and 257 bp product was observed in agarose gel electrophoresis. In specificity test, no product other than the CMV sample was observed. LOD was 100 copy/reaction. All healthy controls were negative, and 33% of the samples were CMV positive.Conclusions: This study shows that CMV can be considered as an infectious and influencing factor in leukemia.

کلیدواژه‌ها [English]

  • Coexistent conditions
  • Cytomegalovirus
  • Leukemia
  • Polymerase chain reaction

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سابقه مقاله

  • تاریخ دریافت: 08 مرداد 1399
  • تاریخ بازنگری: 07 اردیبهشت 1403
  • تاریخ پذیرش: 17 فروردین 1401

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